目次
培養液の調整方法は「培養液の調整」を参照。
培地 / Medium
| DMEM-HG (DMEM) |
Dulbecco's modified Eagle medium 4.5 g/l (25 mM) glucose 4 mM L-glutamine 1 mM sodium pyruvate Phenol red |
ナカライ 08458-45 (1本) 08458-16 (10本) |
4°C |
|---|---|---|---|
| DMEM-LG | Dulbecco's modified Eagle medium 1.0 g/l (5.56 mM) glucose 4 mM L-glutamine 1 mM sodium pyruvate Phenol red |
ナカライ 08456-65 (1本) 08458-36 (10本) |
4°C |
| EMEM | Eagle's minimal essential medium L-glutamine Sodium pyruvate Phenol red |
Wako 055-08975 |
4°C |
| Ham's F-10 (powder) |
Nutrient mixture F-10 Ham L-glutamine NaHCO3 (-) Phenol red |
SIGMA N6635-10X1L |
4°C |
| Ham's F-10 (liquid) |
Nutrient mixture F-10 Ham L-glutamine Sodium pyruvate Phenol red |
Thermo 11550043 (HEPES(-)) 12390035 (HEPES(+)) |
4°C |
| Ham's F-12 | Nutrient mixture F-12 Ham L-glutamine Sodium pyruvate Phenol red |
ナカライ 17458-65 |
4°C |
| RPMI 1640 | RPMI 1640 L-Glutamine Phenol red |
ナカライ 30264-85 (1本) 30264-56 (10本) |
4°C |
血清 / Serum
| FBS | Fetal bovine serum | HyClone SH30910, Lot#AAJ208535 (2015) SH30910, Lot#AC10235246 (2017) |
-20°C | 50 ml ずつ分注 |
|---|---|---|---|---|
| HS | Horse serum | HyClone SH30074.03 |
-20°C | 50 ml ずつ分注 |
サプリメント / Supplement
| AA | Ascorbic acid | Wako 012-04802 |
-20°C | 調整方法 |
|---|---|---|---|---|
| bFGF | Basic fibroblast growth factor, human, recombinant |
Wako 064-04541 |
-80°C | 調整方法 |
| BMP2 | BMP2 human, recombinant |
Wako 026-14811 |
-80°C | 調整方法 |
| BMP4 | BMP4 mouse, recombinant |
Wako 023-18461 |
-80°C | 調整方法 |
| β-GP | β-glycerophosphate | ナカライ 17130-22 |
-20°C | 調整方法 |
| CEE | Chick embryo extract | 岩井化学 / US Biological C3999-20 ml |
-20°C | 5 ml ずつ分注 |
| CHIR | CHIR99021, GSK3β inhibitor |
Wako 034-23103 |
-20°C | 調整方法 |
| DEX | Dexamethasone | Wako 047-18863 |
-20°C | 調整方法 |
| IBMX | 3-isobutyl-1-methylxanthine | Wako 095-03413 |
-20°C | 調整方法 |
| Insulin | Insulin, human, recombinant |
Wako 099-06473 |
4°C -20°C |
調整方法 |
| LIF | Leukemia inhibitory factor, mouse, recombinant |
ナカライ 07695-81 |
4°C | - |
| 2-ME | 2-mercaptoethanol | ナカライ 21438-82 |
4°C | 調整方法 |
| NEAA | MEM non-essential amino acids, ×100 |
Wako 139-15651 |
-20°C | 10 ml ずつ分注 |
| Nucleosides | Nucleosides, ×100 |
Millipore ES-800-D |
-20°C | 10 ml ずつ分注 |
| 4-OHT | 4-hydroxytamoxifen | ナカライ19883-54 | -20°C | 調整方法 |
| PD | PD0325901, MEK1/2 inhibitor |
Wako 162-25291 |
-20°C | 調整方法 |
| PS | Penicillin Streptomycin, stabilized, ×100 |
ナカライ 09367-34 |
-20°C | 10 ml ずつ分注 |
| RANKL | RANKL mouse, recombinant |
abcam ab129136 |
-20°C | 調整方法 |