Backbone pcDNA3.2/V5-DEST
Insert FLAG-mGATA4(K311A/K318A/K320A/K322A)
Seq cDNA / amino acid
Site NcoI/EcoRI
Selection Amp


K311, K318, K320, and K322 of GATA4 are acetylated by p300. GATA4(M456) is lacking p300-induced acetylation, DNA-binding, and transcriptioanl activities. GATA4(M456) serves as a dominant-negative form because it can interact with its partners including p300 (Takaya, 2008).

This plasmid was established by Dr. Yoichi Sunagawa using pcDNA-GATA4(M456) and kindly provided from Hasegawa lab.


Takaya T, Kawamura T, Morimoto T, Ono K, Kita T, Shimatsu A, Hasegawa K. Identification of p300-targeted acetylated residues in GATA4 during hypertrophic responses in cardiac myocytes. J Biol Chem. 2008; 283: 9828-35.