pcDNA-GATA4(M456)
| Backbone | pcDNA3.2/V5-DEST |
|---|---|
| Insert | mGATA4(K311A/K318A/K320A/K322A) |
| Seq | cDNA / amino acid |
| Site | ? |
| Selection | Amp |
Note
K311, K318, K320, and K322 of GATA4 are acetylated by p300. GATA4(M456) is lacking p300-induced acetylation, DNA-binding, and transcriptioanl activities. GATA4(M456) serves as a dominant-negative form because it can interact with its partners including p300 (Takaya, 2008).
This plasmid was established by Tomohide Takaya and kindly provided from Hasegawa lab.
Reference
Takaya T, Kawamura T, Morimoto T, Ono K, Kita T, Shimatsu A, Hasegawa K. Identification of p300-targeted acetylated residues in GATA4 during hypertrophic responses in cardiac myocytes. J Biol Chem. 2008; 283: 9828-35.