TOPO-FLAG-GATA4(S105E)
| Backbone | pCR-BluntII-TOPO |
|---|---|
| Insert | FLAG-mGATA4(S105E) |
| Seq | cDNA / amino acid |
| Site | EcoRI/XhoI |
| Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-EcoRI-FLAG-GATA4(S105E)-XhoI→-EcoRI...←M13 Rev...TOPO
Note
S105 of GATA4 is phosphorylated by ERK1/2 (Liang, 2001) and is important for transcriptional activity (van Berlo, 2011). GATA4(S105E) is expected to mimic phosphorylated GATA4.
FLAG-GATA4(S105E) cDNA was amplified by using pcDNA-FLAG-GATA4(S105E) as a template.
Reference
Liang Q, Wiese RJ, Bueno OF, Dai YS, Markham BE, Molkentin JD. The transcription factor GATA4 is activated by extracellular signal-regulated kinase 1- and 2-mediated phosphorylation of serine 105 in cardiomyocytes. Mol Cell Biol. 2001; 21: 7460-9.
van Berlo JH, Elrod JW, Aronow BJ, Pu WT, Molkentin JD. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2011; 108: 12331-6.