Backbone pCR-BluntII-TOPO
Insert FLAG-mGATA4(S105E)
Seq cDNA / amino acid
Site EcoRI/XhoI
Selection Kan


TOPO...M13 Fwd→...EcoRI-EcoRI-FLAG-GATA4(S105E)-XhoI→-EcoRI...←M13 Rev...TOPO


S105 of GATA4 is phosphorylated by ERK1/2 (Liang, 2001) and is important for transcriptional activity (van Berlo, 2011). GATA4(S105E) is expected to mimic phosphorylated GATA4.

FLAG-GATA4(S105E) cDNA was amplified by using pcDNA-FLAG-GATA4(S105E) as a template.


Liang Q, Wiese RJ, Bueno OF, Dai YS, Markham BE, Molkentin JD. The transcription factor GATA4 is activated by extracellular signal-regulated kinase 1- and 2-mediated phosphorylation of serine 105 in cardiomyocytes. Mol Cell Biol. 2001; 21: 7460-9.

van Berlo JH, Elrod JW, Aronow BJ, Pu WT, Molkentin JD. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2011; 108: 12331-6.