Backbone pcDNA3.2/V5-DEST
Insert FLAG-mGATA4(S105E)
Seq cDNA / amino acid
Site NcoI/EcoRI
Selection Amp


S105 of GATA4 is phosphorylated by ERK1/2 (Liang, 2001) and is important for transcriptional activity (van Berlo, 2011). GATA4(S105E) is expected to mimic phosphorylated GATA4.

This plasmid was established by Dr. Yoichi Sunagawa and kindly provided from Hasegawa lab.


Liang Q, Wiese RJ, Bueno OF, Dai YS, Markham BE, Molkentin JD. The transcription factor GATA4 is activated by extracellular signal-regulated kinase 1- and 2-mediated phosphorylation of serine 105 in cardiomyocytes. Mol Cell Biol. 2001; 21: 7460-9.

van Berlo JH, Elrod JW, Aronow BJ, Pu WT, Molkentin JD. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2011; 108: 12331-6.