Sirius red staining
Material
- Cryosection on slide glass
- Acetone (-20C)
- Sirius Red (1.3% aqueous picric acid/0.1% Direct Red 80)
- 0.5% acetic acid
- EtOH
- Xylene
- DPX
- Cover glass (24x50 mm)
Protocol
- Fix by acetone on ice for 10 min.
- Wash by DW for 1 min.
- Stain collagen fiber with sirius red for 15 min.
- Wash by acetic acid for 1 min.
- Dehydrate by 100% EtOH for 2 min.
- Clear by xylene for 10 min.
- Add 3-4 drop of DPX.
- Encrose by cover glass.