Myogenic differentiation-inducing oligodeoxynucleotide recovers the myoblast differentiation impaired by abnormal glucose concentration
Shunichi Nakamura1, Shinichi Yonekura1,2, Takeshi Shimosato3, Tomohide Takaya1,2.
- Faculty of Agriculture, Shinshu University, Nagano, Japan.
- Institute for Biomedical Sciences, Shinshu University, Nagano, Japan.
- Research Center for Fungal and Microbial Dynamism, Shinshu University, Nagano, Japan.
The 4th International Conference on Pharma-Food (Shizuoka, Japan), 2018/11/15 (Poster).
This study won the Poster Presentation Award.
Abstract
Glucose spike is a set of acute increase and decrease in blood glucose level. Glucose spike is a risk factor for metabolic and cardiovascular disorders which occasionally lead muscle atrophy. However, it has not been reported the effect of glucose spike on myoblasts, which are myogenic progenitor cells maintaining skeletal muscle tissue.
We investigated proliferation and differentiation of the murine C2C12 myoblast cell line in growth and differentiation medium with abnormal glucose concentration. Continuous or oscillated high glucose concentration significantly suppressed both growth and myotube formation of C2C12 cells. Intriguingly, abnormal glucose conditions did not altered MyoD expression but induced myostatin, a myokine inhibiting myogenesis, and suppressed myogenin, a myogenic transcription factor.
Recently, we identified the 18 nt-oligodeoxynucleotide, named myoDN, which intensively facilitates myogenic differentiation. Administration of myoDN to C2C12 cells significantly promoted differentiation into myosin-positive myotubes not only in normal glucose condition but also in continuous or oscillated high glucose concentration. It indicates that myoDN will be useful for prevention or therapy for skeletal muscle atrophy caused by abnormal blood glucose level including glucose spike.