Developmental analysis of the donor mice ES cells in the recipient chick embryo

Developmental analysis of the donor mice ES cells in the recipient chick embryo

Mone Asai1, Tomoya Kusudo1, Kenta Kobayashi1, Koichi Watanabe1, Tamao Ono1, Tomohide Takaya2, Ikuo Tomioka2, Hiroshi Kagami1.

  1. Faculty of Agriculture, Shinshu University, Nagano, Japan.
  2. Institute for Biomedical Sciences, Shinshu University, Nagano, Japan.

2018 International Symposium on Animal Science and Technology (Ina, Japan), 2018/08/10 (Poster).

Abstract

Summary: Generation of mouse-chicken chimeras was challenged. The manipulated chimeric embryos were sampled and fluorescence signals derived from ES cells were observed. A novel stretegy for avian reproductive assist technology, AART, has been developed.

Materials & Methods: GFP-ES cells were injected into chicken blastoderm and the embryos were ex ovo cultured. Some of the embryos were observed under a fluorescent stereoscopic microscope. In vitro cultured embryos of White Leghorn fertilized eggs were prepared. To secure the respiration, the chorioallantoic membrane was secured upward by opening the chorioallantoic membrane where the regression of the blood vessel was confirmed.

Results & Discussion: AART was established by the present studies. It should be an effective methods to the efficient embryonic development toward to hatching. The concentration of ES cells was procured from three groups of 250 cells/ul, 500 cells/ul, and 1000 cells/ul. Although it was confirmed that hatching occurred even at the concentration of 500 cells/ul or less even when mouse ES was injected, incidence was extremely poor in the group of 1000 cells/ul, and hatching could not be confirmed. Furthermore, individuals emitting fluorescence, which seems to be mouse-chicken chimera embryos, were confirmed.