Efficient production of chicks by ex ovo culture system with novel avian assisted reproductive technology (AART)

Efficient production of chicks by ex ovo culture system with novel avian assisted reproductive technology (AART)

Sayami Sato1, Tomoya Sato1, Shizuka Miki1, Tamao Ono1, Tomohide Takaya2, Hiroshi Kagami1.

  1. Faculty of Agriculture, Shinshu University, Nagano, Japan.
  2. Institute for Biomedical Sciences, Shinshu University, Nagano, Japan.

2017 Japan-Thailand International Symposium on Animal Biotechnology (Ina, Japan), 2017/07/05 (Poster).

Abstract

Summary: In chick ex ovo culture system, most of the embryos can be dead basket during a few days just before hatching. If the embryo development to hatching could be assisted by avian reproductive technologies, progress could be contributed for avian biotechnology. In the present studies, a novel method of avian assisted reproductive technology; AART, have been developed for embryo manipulation.

Materials & Methods: Fertilized eggs derived from White Leghorn or Barred Plymouth Rock were used. These embryos were transferred ex ovo culture system. Chimeras by donor stem cell and recipe embryos were prepared in blastodermal stage. These manipulated embryos were ex ovo cultured. Artificial assistance for the embryo just before hatching was performed, in case, autonomous development and hatching would be difficult.

Results & Discussion: Survived until the 20th day of embryo just before hatching. There were individuals, of which were able to release the beak to the air outside the chorioallantoic membrane. They died before switching to lung respiration. For the remaining individuals, the beak reached just below the membrane. Since it had come, some of the membrane was broken by artificial treatment, touch the beak to the air, cut it to the lung breathing. Without any assistance, many of individuals died at this point, but the assisted individuals artificially remove the eggshell increasingly survived until hatching. It was concluded that the novel avian assisted reproductive technology (AART) could dramatically improve the hatchability of the manipulated embryos. The AART should be one of the powerful tools for avian biotechnology.