Purification of primary myoblast by pre-plating

Purification of Primary Myoblast by Pre-plating



  1. Trypisinize cell.
  2. Suspend cell in growth medium.
  3. OPTIONAL: Add 2.5 ug/ml Fungizone (1/100 of stock).
  4. Seed on non-coated culture dish.
  5. Incubate at 37C for 60-90 min.
  6. Check dish whether fibroblast is attaching and myoblast is floating.
  7. Move supernatant (myoblast-rich) to collagen-coated dish.
  8. OPTIONAL: Add growth medium to non-coated dish (fibroblast-rich) to keep remaining myoblast.