Backbone pMXs-IRES-Puro
Insert FLAG-mGATA4(S105A)
Seq cDNA / amino acid
Site EcoRI/XhoI
Selection Amp/Puro


S105 of GATA4 is phosphorylated by ERK1/2 (Liang, 2001). GATA4(S105A) shows reduced transcriptional activity (van Berlo, 2011).

FLAG-GATA4(S105A) cDNA was obtained from TOPO-FLAG-GATA4(S105A) cut by EcoRI and XhoI.


Liang Q, Wiese RJ, Bueno OF, Dai YS, Markham BE, Molkentin JD. The transcription factor GATA4 is activated by extracellular signal-regulated kinase 1- and 2-mediated phosphorylation of serine 105 in cardiomyocytes. Mol Cell Biol. 2001; 21: 7460-9.

van Berlo JH, Elrod JW, Aronow BJ, Pu WT, Molkentin JD. Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2011; 108: 12331-6.