HE (hematoxylin-eosin) staining
HE (hematoxylin-eosin) staiing
Material
- Cryosection on slide glass
- 2% PFA/PBS (4C)
- Hematoxylin
- Eosin
- 95% EtOH
- 100% EtOH
- Xylene
- DPX
- Cover glass (24x50 mm)
Protocol
- Fix by 2% PFA for 5 min.
- Wash by DW for 1 min.
- Stain nuclei with hematoxylin for 1-5 min.
- Wash by tap water for 1 min.
- Wash by DW for 1 min.
- Stain cytoplasm with eosin for 5 min.
- Dehydrate by 95% EtOH for 30 sec.
- Dehydrate by 100% EtOH for 2 min.
- Dehydrate by another 100% EtOH for 2 min.
- Clear by xylene for 10 min.
- Add 3-4 drop of DPX.
- Enclose by cover glass.
