pMX-puro-M3-GATA4
| Backbone | pMXs-IRES-Puro |
|---|---|
| Insert | mM3-mGATA4 |
| Selection | Amp/Puro |
Note
The transactivation domain of mouse MyoD(1-62) (M3) was fused to N-terminus of mouse GATA4 (Hirai, 2013). It has not been confirmed that M3-GATA4 actually has the enhanced transcriptional activity compared with intact GATA4.
This plasmid was kindly provided from Kikyo lab.
Reference
Hirai H, Katoku-Kikyo N, Keirstead SA, Kikyo N. Accelerated direct reprogramming of fibroblasts into cardiomyocyte-like cells with the MyoD transactivation domain. Cardiovasc Res. 2013; 100: 105-13.