TOPO-GATA5
Backbone | pCR-BluntII-TOPO |
---|---|
Insert | mGATA5(M383L) |
Seq | cDNA / amino acid |
Site | EcoRI/XhoI |
Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-EcoRI-GATA5(M383L)-XhoI→-EcoRI...←M13 Rev...TOPO
Note
GATA5 cDNA was amplified by using pGATA5 plasmid from Hasegawa lab as a template. pGATA5 has a polymorphism M383L (A1147C), which is corresponded to rat GATA5. The region including this amino acid is not conserved in human.