TOPO-GATA4-3'UTR
Backbone | pCR-BluntII-TOPO |
---|---|
Insert | mGATA4-3'UTR |
Seq | cDNA |
Site | XbaI (GATA4-3'UTR has a XbaI site) |
Selection | Kan |
Insertion
TOPO...M13 Fwd→...EcoRI-XbaI-GATA4-3'UTR-XbaI→-EcoRI...←M13 Rev...TOPO
Note
GATA4-3'UTR cDNA was amplified by using mouse heart cDNA as a template. GATA4-3'UTR has a XbaI site. Use TOPO-GATA4-3'UTR-SpeI for inserting GATA4-3'UTR into XbaI site.