Plasmid

Index

GATA factor

Name	Backbone	Insert	Selection
pcDNA-FLAG-GATA4	pcDNA3.2/V5-DEST	FLAG-mGATA4	Amp
pcDNA-HA-GATA4	pcDNA3.2/V5-DEST	HA-mGATA4	Amp
pcDNA-GATA4(M456)	pcDNA3.2/V5-DEST	mGATA4(K311A/K318A/K320A/K322A)	Amp
pcDNA-FLAG-GATA4(M456)	pcDNA3.2/V5-DEST	FLAG-mGATA4(K311A/K318A/K320A/K322A)	Amp
pcDNA-FLAG-GATA4(S105A)	pcDNA3.2/V5-DEST	FLAG-mGATA4(S105A)	Amp
pcDNA-FLAG-GATA4(S105D)	pcDNA3.2/V5-DEST	FLAG-mGATA4(S105D)	Amp
pcDNA-FLAG-GATA4(S105E)	pcDNA3.2/V5-DEST	FLAG-mGATA4(S105E)	Amp
pMX-puro-GATA1	pMXs-IRES-Puro	mGATA1	Amp/Puro
pMX-puro-GATA2	pMXs-IRES-Puro	mGATA2	Amp/Puro
pMX-puro-GATA3	pMXs-IRES-Puro	mGATA3	Amp/Puro
pMX-puro-FLAG-GATA4	pMXs-IRES-Puro	FLAG-mGATA4	Amp/Puro
pMX-puro-FLAG-GATA4(Kmut)	→ pMX-puro-FLAG-GATA4(M456)
pMX-puro-FLAG-GATA4(M456)	pMXs-IRES-Puro	FLAG-mGATA4(K311A/K318A/K320A/K322A)	Amp/Puro
pMX-puro-FLAG-GATA4(S105A)	pMXs-IRES-Puro	FLAG-mGATA4(S105A)	Amp/Puro
pMX-puro-FLAG-GATA4(S105D)	pMXs-IRES-Puro	FLAG-mGATA4(S105D)	Amp/Puro
pMX-puro-FLAG-GATA4(S105E)	pMXs-IRES-Puro	FLAG-mGATA4(S105E)	Amp/Puro
pMX-puro-M3-GATA4	pMXs-IRES-Puro	mM3-mGATA4	Amp/Puro
pMX-puro-GATA5	pMXs-IRES-Puro	mGATA5(M383L)	Amp/Puro
pMX-puro-GATA6	pMXs-IRES-Puro	mGATA6	Amp/Puro
TOPO-GATA1	pCR-BluntII-TOPO	mGATA1	Kan
TOPO-GATA2	pCR-BluntII-TOPO	mGATA2	Kan
TOPO-GATA3	pCR-BluntII-TOPO	mGATA3	Kan
TOPO-FLAG-GATA4	pCR-BluntII-TOPO	FLAG-mGATA4	Kan
TOPO-FLAG-GATA4(M456)	pCR-BluntII-TOPO	FLAG-mGATA4(K311A/K318A/K320A/K322A)	Kan
TOPO-FLAG-GATA4(S105A)	pCR-BluntII-TOPO	FLAG-mGATA4(S105A)	Kan
TOPO-FLAG-GATA4(S105D)	pCR-BluntII-TOPO	FLAG-mGATA4(S105D)	Kan
TOPO-FLAG-GATA4(S105E)	pCR-BluntII-TOPO	FLAG-mGATA4(S105E)	Kan
TOPO-GATA5	pCR-BluntII-TOPO	mGATA5(M383L)	Kan
pUCIDT-GATA6	pUCIDT-KAN	mGATA6	Kan

p300

pCMVβ-p300-HA	?	hp300-HA	Amp
pCMVβ-p300WY-HA	?	hp300(W1466S/Y1467A)-HA	Amp
pcDNA-FLAG-p300(1514-1922)	pcDNA3.2/V5-DEST	FLAG-hp300(1514-1922)	Amp
pcDNA-HA-p300(1514-1922)	pcDNA3.2/V5-DEST	HA-hp300(1514-1922)	Amp
pMX-puro-HA-p300(1514-1922)	pMXs-IRES-Puro	HA-hp300(1514-1922)	Amp/Puro
TOPO-HA-p300(1514-1922)	pCR-BluntII-TOPO	HA-hp300(1514-1922)	Kan

UTR

pGL3-Luc-GATA4-3'UTR	pGL3-Luc-Control	mGATA4-3'UTR	Amp
pGL3-Luc-GATA4-3'UTR-miR26mut	pGL3-Luc-Control	mGATA4-3'UTR()	Amp
pGL3-Luc-GATA4-3'UTR-miR200del	pGL3-Luc-Control	mGATA4-3'UTR()	Amp
pGL3-Luc-GATA4-3'UTR-miR208mut	pGL3-Luc-Control	mGATA4-3'UTR()	Amp
TOPO-GATA4-3'UTR	pCR-BluntII-TOPO	mGATA4-3'UTR	Kan
TOPO-GATA4-3'UTR-SpeI	pCR-BluntII-TOPO	mGATA4-3'UTR	Kan
TOPO-GATA4-3'UTR-miR26mut	pCR-BluntII-TOPO	mGATA4-3'UTR()	Kan
TOPO-GATA4-3'UTR-miR200del	pCR-BluntII-TOPO	mGATA4-3'UTR()	Kan
TOPO-GATA4-3'UTR-miR208mut	pCR-BluntII-TOPO	mGATA4-3'UTR()	Kan

Luciferase

ANF-Luc	?	rANF promoter (-170 bp) ?	Amp
βMHC-Luc	?	rβMHC/Myh7 promoter (-333~+44) ?	Amp
4RSV-Luc	?	4 x E-box	Amp
pGL3-Bcl-2-Luc	pGL3-Luc	Bcl-2 promoter	Amp
pGL3-Bcl-xL-Luc	pGL3-Luc	Bcl-xL promoter	Amp
pGL3-Ccnd2-Luc	pGL3-Luc	mCyclin D2/Ccnd2 promoter	Amp
pGL3-Ccnd2-E1mut-Luc	pGL3-Luc	mCyclin D2/Ccnd2 promoter	Amp
pGL3-Ccnd2-E2mut-Luc	pGL3-Luc	mCyclin D2/Ccnd2 promoter	Amp
pGL3-Ccnd2-E3mut-Luc	pGL3-Luc	mCyclin D2/Ccnd2 promoter	Amp
pGL3-Myl1-Luc	pGL3-Luc	rMLC3f/Myl1 enhancer	Amp
pGL3-Myl1-GATAmut-Luc	pGL3-Luc	rMLC3f/Myl1 enhancer	Amp
pMX-Luc	?	Firefly luciferase	Amp