Index
Nuclear / Cytoplasmic / Membrane/ECM / Secondary
Nuclear protein
| Name/Clone | Company | Host | Immunogen | Cross | Dilution | Reference |
|---|---|---|---|---|---|---|
| GATA2 CG2-96 |
Santa Cruz sc-267 |
Mouse mono |
m | 1/50: PFA-MB ND: PFA-myofiber |
||
| GATA3 HG3-31 |
Santa Cruz sc-268 |
Mouse mono |
h-GATA3 | m | 1/50: PFA-MB ND: PFA-myofiber |
|
| GATA4 C-20 |
Santa Cruz sc-1237 |
Goat poly |
m-GATA4 C-terminus |
m | IP, WB 1/500: PFA-MB ND: PFA-myofiber ND: PFA-Skm |
Takaya, 2008 |
| GATA4 G-4 |
Santa Cruz sc-25310 |
Mouse mono |
h-GATA4 328-439 aa |
Bad: PFA-mES Bad: PFA-MB |
||
| GATA4 H-112 |
Santa Cruz sc-9053 |
Rabbit poly |
h-GATA4 328-439 aa |
m | IP, WB Bad: PFA-mES Bad: PFA-MB |
Takaya, 2008 |
| GATA4 ERP4768 |
abcam ab134057 |
Rabbit mono |
GATA4 N-terminus |
Bad: PFA-mES Bad: PFA-MB |
||
| GATA4 eBioEvan |
eBioscience 14-9980 |
Rat mono |
m | 1/1000: PFA-mES 1/500: PFA-MB 1/500: PFA-myofiber |
||
| GATA6 C-20 |
Santa Cruz sc-7244 |
Goat poly |
h-GATA6 C-terminus |
Bad: PFA-MB | ||
| GATA6 H-92 |
Santa Cruz sc-9055 |
Rabbit poly |
h-GATA6 358-449 aa |
m | IP, WB Bad: ChIP-MB Bad: PFA-mES Bad: PFA-MB Bad: PFA-myofiber |
Takaya, 2008 |
| GATA6 N-18 |
Santa Cruz sc-7245 |
Goat poly |
h-GATA6 N-terminus |
Bad: PFA-MB | ||
| GATA6 D61E4 |
Cell Signaling 5851 |
Rabbit mono |
h-GATA6 N-terminus |
m | 1/1000: PFA-mES 1/1000: PFA-MB 1/1000: PFA-myofiber |
|
| Myogenin F5D |
DSHB F5D |
Mouse mono |
r-Myogenin | m | 1/10: PFA-mES 1/10: PFA-MB 1/10: PFA-myofiber |
|
| Myogenin M-225 |
Santa Cruz sc-576 |
Rabbit poly |
r-Myogenin | m | 1/500: PFA-MB | |
| MyoD M-318 |
Santa Cruz sc-760 |
Rabbit poly |
m-MyoD | m | ChIP 1/500: PFA-MB 1/500: PFA-myofiber |
|
| P-histone H3 (S10) D2C8 |
Cell Signaling 3377 |
Rabbit mono |
h-Histone H3 (S10) phospho peptide |
m | 1/1000: PFA-MB | |
| Pax7 PAX7 |
R&D MAB1675 |
Mouse mono |
ch-Pax7 352-523 aa |
m | 1/200: PFA-mES 1/200: PFA-MB 1/200: PFA-myofiber |
Cytoplasmic protein
| MHC (embryonic) F1.652 |
DSHB F1.652 |
Mouse mono |
h-Myosin | m | 1/10: Acetone-Skm | |
| MHC (sarcomera) MF20 |
DSHB MF20 |
Mouse mono |
ch-Myosin | m | 1/20: PFA-MB |
Membrane/ECM protein
| CD31-PE 390 |
eBioscience 12-0311 |
Rat mono |
m | 1/200: MACS-Skm | Motohashi, 2014 | |
| CD45-PE 30-F11 |
eBioscience 12-0451 |
Rat mono |
m | 1/200: MACS-Skm | Motohashi, 2014 | |
| Flk1-PE Avas12a1 |
eBioscience 12-5821 |
Rat mono |
m | 1/200: FACS-mES | ||
| Integrin α7 3C12 |
MBL K0046-3 |
Mouse mono |
Mouse MB |
m | 1/200: MACS-Skm | Motohashi, 2014 |
| Laminin | Sigma L9393 |
Rabbit poly |
m-sarcomeric laminin |
m | 1/500: Acetone-Skm | |
| Laminin 4H8-2 |
Sigma L0663 |
Rat mono |
m-heart laminin-2 |
m | 1/500: Acetone-Skm | |
| PDGFRα-APC APA5 |
BD 562777 |
Rat mono |
m-PDGFRα | m | 1/200: FACS-mES 1/200: MACS-mES Bad: PFA-mES |
|
| Sca1-PE D7 |
eBioscience 12-5981 |
Rat mono |
m | 1/200: MACS-Skm | Motohashi, 2014 |
Secondary antibody
| @mIgG-MicroBeads | Miltenyi Biotec 130-048-401 |
Goat | mIgG1, mIgG2a, mIgG2b, mIgG3 |
1/20 | Motohashi, 2014 | |
| @APC-MicroBeads | Miltenyi Biotec 130-090-855 |
Mouse mono |
1/20 | |||
| @Biotin-MicroBeads | Miltenyi Biotec 130-090-485 |
Mouse mono |
1/20 | |||
| @FITC-MicroBeads | Miltenyi Biotec 130-048-701 |
Mouse mono |
1/20 | |||
| @PE-MicroBeads | Miltenyi Biotec 130-048-801 |
Mouse mono |
1/20 | Motohashi, 2014 |